CaltechTHESIS
  A Caltech Library Service

Bioanalytical Tools to Develop Rapid Diagnostics and Study Physiology

Citation

Rolando, Justin Charles (2019) Bioanalytical Tools to Develop Rapid Diagnostics and Study Physiology. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/99NT-WP75. https://resolver.caltech.edu/CaltechTHESIS:05292019-112017023

Abstract

This work details the development of bioanalytical tools for use in rapid diagnostics (Chapters 2-4) and in the study of physiology (Chapters 5-6). This research harness the power of real-time, singlemolecule microfluidics to study loop-mediated isothermal amplification in urinary tract infections (Chapter 2), chlamydia (Chapter 3), and gonorrhea (Chapter 4). In Chapter 5, non-reactive beads are designed and optimized to study the impact of polymers on murine gastrointestinal mucosa. Chapter 6 details the implementation of a mass spectrometry method to quantify bile acids and investigate their interaction with the microbiota in the murine gastro-intestinal tract.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Bioanalytical, Diagnostics, Physiology, loop-mediated isothermal amplification, urinary tract infections, chlamydia, gonorrhea, murine gastrointestinal mucosa, bile acids
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Ismagilov, Rustem
Thesis Committee:
  • Tirrell, David A. (chair)
  • Kornfield, Julia A.
  • Grubbs, Robert H.
  • Heath, James R.
  • Ismagilov, Rustem F.
Defense Date:10 May 2019
Funders:
Funding AgencyGrant Number
Defense Threat Reduction Agency (DTRA)MCDC-18-01-01-00
Burroughs Wellcome FundInnovation in Regulatory Science Award
Defense Advanced Research Projects Agency (DARPA)HR0011-15-C-009
Defense Advanced Research Projects Agency (DARPA)HROO 11-17-2-0037
Defense Advanced Research Projects Agency (DARPA)HR0011-11-2-0006
Defense Advanced Research Projects Agency (DARPA)HROO1 1-17-2-0064
Joseph J. Jacobs Institute for Molecular Engineering for MedicineUNSPECIFIED
Record Number:CaltechTHESIS:05292019-112017023
Persistent URL:https://resolver.caltech.edu/CaltechTHESIS:05292019-112017023
DOI:10.7907/99NT-WP75
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/acs.analchem.8b04324DOIArticle adapted for Ch. 2
https://doi.org/10.7554/eLife.40387.001DOIArticle adapted for Ch. 5
ORCID:
AuthorORCID
Rolando, Justin Charles0000-0001-8948-319X
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11559
Collection:CaltechTHESIS
Deposited By: Justin Rolando
Deposited On:01 Jun 2019 00:48
Last Modified:10 Feb 2020 16:57

Thesis Files

[img]
Preview
PDF - Final Version
See Usage Policy.

8MB

Repository Staff Only: item control page