From monoclonal antibody to gene for a neuron-specific glycoprotein in Drosophila
Abstract
A monoclonal antibody (MAb24B10), derived from mice immunized with Drosophila retina, exclusively stains photoreceptor cells in the retina and their axonal projections to the optic ganglia. The antigen (Ag24B10) is a 160-kDa glycoprotein comprising about 0.8% of the retina protein. By microsequencing, 19 of the first 21 amino acids at the NH2-terminal end of the protein have been determined. Using synthetic oligonucleotide probes corresponding to a portion of this amino acid sequence, we isolated a homologous genomic clone. A partial DNA sequence of this clone, along with blot experiments on genomic DNA and RNA, indicate that this clone is part of the structural gene for Ag24B10. By in situ hybridization, the gene was localized to the tip of chromosome 3R.
Additional Information
© 1985 by the National Academy of Sciences. Contributed by Seymour Benzer, November 8, 1984. We thank Drs. Dennis Ballinger, Elliot Meyerowitz, and David Teplow for helpful discussion and, in particular, Dr. Meyerowitz for generously providing laboratory facilities. This work was supported by the Helen Hay Whitney Foundation (S.L.Z.), the Gosney Foundation (T.R.V.), and National Science Foundation Grant PCM 79-11771 (S.B.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.Attached Files
Published - ZIPpnas85.pdf
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Additional details
- PMCID
- PMC397374
- Eprint ID
- 10078
- Resolver ID
- CaltechAUTHORS:ZIPpnas85
- Helen Hay Whitney Foundation
- Gosney Foundation
- PCM 79-11771
- NXSF
- Created
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2008-04-10Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field