Published October 1, 2000
| public
Journal Article
Open
Action of the Thiamine Antagonist Bacimethrin on Thiamine Biosynthesis
Chicago
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Abstract
Bacimethrin is an analog of the 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) moiety of thiamine and inhibits the growth of Salmonella enterica serovar Typhimurium on a defined medium. Two classes of mutants that had increased bacimethrin resistance were isolated and characterized. Results showed that overexpression of the thi operon or specific lesions in thiD resulted in a bacimethrin-resistant phenotype. Phenotypic analyses of the thiD mutants suggested that they had a specific defect in one of the two kinase activities associated with this gene product and, further, that ThiD and not PdxK was primarily responsible for salvage of HMP from the medium.
Additional Information
Copyright © 2000, American Society for Microbiology. Received 17 April 2000/Accepted 17 July 2000 We thank T. Begley for providing us with bacimethrin and for helpful discussion and S. Weber for technical assistance. Plasmid pVJS717 was obtained from V. Stewart. This work was supported by competitive grant MCB9723830 from the National Science Foundation and a Shaw Scientists Award from the Milwaukee Foundation. J.L.Z. was supported by a National Science Foundation Graduate Fellowship and a Wisconsin Alumni Research Foundation Annual Fellowship.Files
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