The RNA–protein complex: Direct probing of the interfacial recognition dynamics and its correlation with biological functions
Abstract
The N protein from bacteriophage {lambda} is a key regulator of transcription antitermination. It specifically recognizes a nascent mRNA stem loop termed boxB, enabling RNA polymerase to read through downstream terminators processively. The stacking interaction between Trp-18 of WT N protein and A7 of boxB RNA is crucial for efficient antitermination. Here, we report on the direct probing of the dynamics for this interfacial binding and the correlation of the dynamics with biological functions. Specifically, we examined the influence of structural changes in four peptides on the femtosecond dynamics of boxB RNA (2-aminopurine labeled in different positions), through mutations of critical residues of N peptide (residues 1–22). We then compare their in vivo (Escherichia coli) transcription antitermination activities with the dynamics. The results demonstrate that the RNA–peptide complexes adopt essentially two dynamical conformations with the time scale for interfacial interaction in the two structures being vastly different, 1 ps for the stacked structure and nanosecond for the unstacked one; only the weighted average of the two is detected in NMR by nuclear Overhauser effect experiments. Strikingly, the amplitude of the observed ultrafast dynamics depends on the identity of the amino acid residues that are one helical turn away from Trp-18 in the peptides and is correlated with the level of biological function of their respective full-length proteins.
Additional Information
© 2003 National Academy of Sciences. Contributed by Ahmed H. Zewail, May 22, 2003. This work was financially supported by the National Science Foundation. H.-C. B. is supported in part by the Wenner-Gren Foundation in Stockholm.Attached Files
Published - XIApnas03.pdf
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Additional details
- PMCID
- PMC166192
- Eprint ID
- 497
- Resolver ID
- CaltechAUTHORS:XIApnas03
- NSF
- Wenner-Gren Foundation
- Created
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2005-07-11Created from EPrint's datestamp field
- Updated
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2023-06-01Created from EPrint's last_modified field