Published August 1974
| Published
Journal Article
Open
A DNA-Protein Complex Involved in Bacteriophage phi X174 Particle Formation
- Creators
- Weisbeek, Peter J.
- Sinsheimer, Robert L.
Chicago
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Abstract
A phi X-specific DNA-protein complex has been isolated from phi X-infected cells. This complex contains infective circular single-stranded DNA, the proteins of the genes F, G, H, and J in the same proportions as in the phage particle, and, in addition, the gene D-protein. The D-protein makes up 12-22% of the protein part of the complex. The sedimentation value of the complex is about 140 S. In vitro, the complex can be converted to the normal 114S phage particle (with a concomitant loss of its D-protein) or to an uninfective 70S particle and a small amount of free single-stranded DNA. The fast sedimenting particle is not associated with membranes.
Additional Information
Copyright © 1974 by the National Academy of Sciences Contributed by Robert L. Sinsheimer, May 16, 1974 This research was supported by a NATO Science Fellowship given to P.J.W. by the Netherlands Organization for the Advancement of Pure Research (Z.W.O.) and by Grant GM 13554 from the United States Public Health Service.Attached Files
Published - WEIpnas74.pdf
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Additional details
- PMCID
- PMC388619
- Eprint ID
- 6231
- Resolver ID
- CaltechAUTHORS:WEIpnas74
- North Atlantic Treaty Organization (NATO)
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO)
- U.S. Public Health Service (USPHS)
- GM 13554
- Created
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2006-11-29Created from EPrint's datestamp field
- Updated
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2019-10-02Created from EPrint's last_modified field