Physical properties of chemically acetylated rat liver chromatin

Creators: Wallace, R. Bruce; Sargent, Thomas D.; Murphy, Robert F.; Bonner, James

Abstract

The physical properties of rat liver chromatin and nucleosomes acetylated with acetic anhydride were examined in order to clarify the mechanism by which chemical acetylation of histones increases template activity in vitro [Marushige, K. (1976) Proc. Natl. Acad. Sci. USA 73, 3937-3941]. Acetylation was found to have dramatic effects on the magnesium solubility, nuclease sensitivity, thermal denaturation, and sedimentation of chromatin and nucleosomes. The significance of the results to models of gene activation and chromatin replication is considered.

Additional Information

© 1977 by the National Academy of Sciences. Contributed by James Bonner, May 23, 1977. We thank Dr. S.K. Dube for many stimulating discussions and critical evaluation of this work and Ms. M.J. Johnson for valuable technical assistance. This work was supported by U.S. Public Health Service Grant GM-13762. R.B.W. is the holder of a Medical Research Council of Canada fellowship. T.D.S. is supported by a predoctoral fellowship from the National Science Foundation. The costs of publication of this article were defrayed in part by the payment of page charges from funds made available to support the research which is the subject of the article. This article must therefore by hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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