Fast fluorescence microscopy for imaging the dynamics of embryonic development
Abstract
Live imaging has gained a pivotal role in developmental biology since it increasingly allows real-time observation of cell behavior in intact organisms. Microscopes that can capture the dynamics of ever-faster biological events, fluorescent markers optimal for in vivo imaging, and, finally, adapted reconstruction and analysis programs to complete data flow all contribute to this success. Focusing on temporal resolution, we discuss how fast imaging can be achieved with minimal prejudice to spatial resolution, photon count, or to reliably and automatically analyze images. In particular, we show how integrated approaches to imaging that combine bright fluorescent probes, fast microscopes, and custom post-processing techniques can address the kinetics of biological systems at multiple scales. Finally, we discuss remaining challenges and opportunities for further advances in this field.
Additional Information
© 2008 HFSP Publishing. Received 14 January 2008; accepted 19 March 2008; published 13 May 2008. We thank Elaine Bearer for providing us with the squid giant axon data used in Fig. 4. We thank Willy Supatto, Thai Truong, and members of the Biological Imaging Center, Beckman Institute at Caltech for discussions and comments. M.L. received support from the Swiss National Science Foundation (Fellowship PA002-111433) and J.V. from the Human Frontier Science Program (HFSP). This work was also supported by a NIH/NICHD grant (PO1HD037105).Attached Files
Published - VERhfspj08.pdf
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Additional details
- PMCID
- PMC2645566
- Eprint ID
- 11528
- Resolver ID
- CaltechAUTHORS:VERhfspj08
- Swiss National Science Foundation (SNSF)
- PA002-111433
- Human Frontier Science Program
- NIH
- PO1HD037105
- Created
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2008-08-31Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field