Published April 1, 1962
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Journal Article
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Interaction of Nucleosides and Related Compounds with Nucleic Acids as Indicated by the Change of Helix-Coil Transition Temperature
- Creators
- T'so, Paul O. P.
- Helmkamp, G. K.
- Sander, C.
Abstract
A series of compounds has been tested for effectiveness in lowering the melting temperature of poly A and of thymus DNA. The order of increasing activity was found to be: adonitol, methyl riboside (both negligible) < cyclohexanol < phenol, pyrimidine, uridine < cytidine, thymidine < purine, adenosine, inosine, deoxyguanosine < caffeine, coumarin, 2,6-dichloro-7-methylpurine. Urea was ineffective with poly A and only slightly effective with DNA. At a concentration of 0.3 M, purine lowered the Tm of DNA about 9°.
Additional Information
Copyright © 1962 by the National Academy of Sciences Communicated by James Bonner, February 27, 1962 We are grateful to Prof. James Bonner for his support in this work and to Prof. N. Davidson and Dr. J. Vinograd for their suggestions concerning the manuscript. The research was supported in part by grants no. RG-3977, no. RG-5143, and no. RG-8185(C1), National Institutes of Health.Files
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