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Published June 1977 | Published
Journal Article Open

Isolation of folded chromosomes from Mycoplasma hyorhinis

Abstract

Folded chromosomes were isolated from Mycoplasma hyorhinis. When examined by electron microscopy, these molecules show variability of loop size, number of loops, total contour length and degree of twisting of the DNA. Sedimentation velocity was unaltered after treatment with RNase, proteinase K, SDS, temperatures up to 65°C and NaCl concentrations from 0.1 M to 4 M.

Additional Information

© 1977 Oxford University Press. Received February 1, 1977. We are grateful to Dr. Harumi Kasamatsu for valuable discussions and suggestions. This work was supported by National Institutes of Health grant CA08014. This is contribution No. 5502 from the Division of Chemistry and Chemical Engineering.

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