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Published August 1, 1978 | public
Journal Article Open

Purification of a DNA nicking-closing enzyme from mouse L cells

Tang, David

Abstract

A DNA nicking-closing enzyme has been purified from the nuclei of souse L cells to 90% homogeneity. The denatured and reduced form of the enzyme has a molecular weight of 68,000 which is In agreement with the molecular weight of the native enzyme as determined by gel filtration and by sucrose sedimentation velocity assuming the protein is globular. Therefore, the active form of the enzyme is a monopolypeptide. Its isoelectric. point is pH 4.2 ± 0.2. The nicking-closing activity does not require a cofactor arid does not involve any sulfhydryl group. The enzyme requires 0.2 M NaCl and pH in the range of 6.5–7.5 for optimal activity.

Additional Information

Copyright © 1978 Oxford University Press. Received April 20, 1978. This work was supported by Grants CA 08014 and GM 15327 from the United States Public Health Service to Dr. Jerome Vinograd. Thanks are due to Judith Campbell, Norman Davidson, and Tom Maniatis for their critical review of the mauscript.

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August 22, 2023
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October 16, 2023