Published August 1967
| Published
Journal Article
Open
Characterization of an Infectivity Assay for the Ribonucleic Acid of Bacteriophage MS2
Chicago
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Abstract
An infectivity assay for MS2 ribonucleic acid (RNA), which uses bacterial spheroplasts of an F– strain of Escherichia coli, has an efficiency of 10^–5 infected spheroplasts per RNA molecule. The characteristics of this assay and the influence of several parameters are presented. Important variables include the duration of exposure of the cells to lysozyme-ethylenediaminetetraacetic acid, the duration of exposure of the spheroplast stock to the RNA solutions before dilution, the concentration of RNA, and the presence of competing RNA. The growth kinetics of the virus in the infected spheroplasts and the extent of lysis have also been studied.
Additional Information
Copyright © 1967 American Society for Microbiology. Received for publication 24 April 1967. We thank Gloria Davis for competent assistance with some of the infectivity assays. We would also like to thank E.R. Glowacki for her aid and inspiration. This investigation was supported by Public Health Service research grant GM 13554. The results presented here are part of a thesis by James H. Strauss, Jr., submitted to the California Institute of Technology in partial fulfillment of the requirements for the Ph.D. degree. http://resolver.caltech.edu/CaltechETD:etd-10102002-121043Attached Files
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Additional details
- PMCID
- PMC375345
- Eprint ID
- 12362
- Resolver ID
- CaltechAUTHORS:STRAjvir67
- NIH
- GM13554
- Created
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2008-11-13Created from EPrint's datestamp field
- Updated
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2019-10-03Created from EPrint's last_modified field