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Published August 1969 | public
Journal Article Open

Ribonucleic acid synthesis of vesicular stomatitis virus. I. Species of Ribonucleic Acid Found in Chinese Hamster Ovary Cells Infected with Plaque-forming and Defective Particles

Abstract

Plaque-forming B particles of vesicular stomatitis virus (VSV) induce the synthesis of virus-specific ribonucleic acid (RNA) in Chinese hamster ovary cells, whereas defective T particles do not. Infection with low input multiplicities of B results in the formation of four species of RNA. During infection with high multiplicities, RNA synthesis begins with mainly these four species of RNA but gradually shifts to a new pattern of RNA synthesis involving five other species of RNA. The change can also be induced by superinfection with T at 2.5 hr after infection with a low multiplicity of B. T added at the same time as B prevents virtually all RNA synthesis. Synthesis of the first group of RNA species correlates with the formation of B particles, whereas synthesis of the second group correlates with the formation of T particles. The various species of RNA formed after infection with VSV particles include single-stranded RNA, a completely double-stranded RNA, and RNA with partially double-stranded regions. These observations begin to establish a molecular basis for understanding the ability of T particles to interfere with the growth of B particles.

Additional Information

Copyright © 1969 by the American Society for Microbiology. Received for publication 9 June 1969. This work was supported by Public Health Service grant AI-08388 from the National Institute of Allergy and Infectious Diseases and by American Cancer Society Research Grant E-512. M.S. is a recipient of a predoctoral fellowship from the National Science Foundation, D.B. received a Faculty Research Award from the American Cancer Society, and A.S.H. was a recipient of a postdoctoral fellowship from the Public Health Service (F2-AI-35,110). We thank Frensch Niegermeier for his critical reading of the manuscript, and Dianne Jack and Donna Smoler for technical assistance.

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August 21, 2023
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October 16, 2023