Published November 1974
| Published
Journal Article
Open
Nucleotide sequences of the 5' termini of øX174 mRNAs synthesized in vitro
Chicago
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Abstract
When using øX17U RFI DNA as a template, in vitro, E. coli RNA polymerase synthesizes four major purine triphosphate-containing 5' end sequences. RNase A digests of 32P labeled RNA were further digested with spleen exonuclease to remove the bulk of the oligonucleotides with 5' hydroxyls and then chromatographed on DEAE cellulose to resolve the remaining 5' terminal oligonucleotides. By application of standard separation and sequence techniques, the major 5' end sequences were shown to be: pppApUp(Cp), pppApApApUp(Cp), pppApApApApUp(Cp), and pppGpApUp(Gp).
Additional Information
Copyright © 1974 Oxford University Press. Received August 20, 1974. The authors acknowledge the excellent technical assistance of Charles Romeo. This research was supported by grant number GM 13554 from the National Institutes of Health.Attached Files
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Additional details
- PMCID
- PMC343428
- Eprint ID
- 4012
- Resolver ID
- CaltechAUTHORS:SMInar74
- U.S. Public Health Service (USPHS)
- GM 13554
- Created
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2006-07-24Created from EPrint's datestamp field
- Updated
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2019-10-02Created from EPrint's last_modified field