Isolation and structure of pro-somatostatin: A putative somatostatin precursor from pig hypothalamus

Creators: Schally, Andrew V.; Huang, Wei-Yong; Chang, Robert C. C.; Arimura, Akira; Redding, Tommie W.; Millar, Robert P.; Hunkapiller, M. W.; Hood, L. E.

Abstract

An octacosapeptide that we named pro-somatostatin has been isolated from acid extracts of porcine hypothalami and found to have the amino acid sequence Ser-Ala-Asn-Ser-Asn-Pro-Ala-Met-Ala-Pro-Arg-Glu-Arg-Lys-Ala-Gly- overline{Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys}. This octacosapeptide possesses high somatotropin (growth hormone) and prolactin release-inhibiting activity in vitro. It also crossreacts strongly with antisera generated against the somatostatin tetradecapeptide. This octacosapeptide is most likely a precursor (pro-hormone) of somatostatin in the hypothalamus. The existence of still larger molecular size precursors of somatostatin was also observed.

Additional Information

© 1980 National Academy of Sciences. Contributed by Andrew V. Schally, April 24, 1980. It is a pleasure to acknowledge the participation of Drs. A. Dupont, H. Matsuo, K. Kangawa, and Raquel Fernandez-Durango in the early stages of this work. This work was supported in part by grants from the Veterans Administration and by U.S. Public Health Service Grants AM-07467 (to A.V.S.), AM-09094 (to A.A.), and GM-06965 (to L.E.H.). R.P.M. was a recipient of a Fellowship from the Medical Research Council of South Africa. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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