A Poliovirus Temperature-Sensitive RNA Synthesis Mutant Located in a Noncoding Region of the Genome

Creators: Sarnow, Peter; Bernstein, Harris D.; Baltimore, David

Abstract

We have constructed an 8-base-pair insertion mutation in the 3' noncoding region of an infectious poliovirus cDNA clone that gives rise to a temperature-sensitive RNA synthesis mutant upon transfection into mammalian cells. The mutated cDNA was used to establish a cell line that releases the mutant poliovirus in a temperature-dependent fashion, representing a unique persistent viral infection. A poliovirus mutant mapping in the noncapsid region of the viral genome can be complemented in this cell line, implying that the cell line expresses viral proteins at the nonpermissive temperature.

Additional Information

© 1986 by the National Academy of Sciences Contributed by David Baltimore, September 9, 1985 We thank Karla Kirkegaard for many valuable suggestions during the course of this work and for helpful comments on the manuscript. We also thank Mike Gilman for critical reading of this manuscript. This work was supported by grants from the National Institute for Allergy and Infectious Diseases. P.S. was supported by a postdoctoral fellowship from the Deutsche Forschungsgemeinschaft. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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