Published November 1969
| Published
Journal Article
Open
Circular Deoxyribonucleic Acid from Shigella dysenteriae Y6R
- Creators
- Rush, Mark G.
- Gordon, C. N.
- Warner, Robert C.
Abstract
Circular deoxyribonucleic acid was isolated from Shigella dysenteriae Y6R and was found to consist of six species having molecular weights of 10^6, 1.3 x 10^6, 2.6 x 10^6, 3.8 x 10^6, 20 x 10^6, and 24 x 10^6 daltons. These size classes were partially resolved by sucrose density gradient centrifugation. The minicircles (10^6 and 1.3 x 10^6) were found to have a buoyant density in CsCl of 1.710 g/ml. The 3.8 x 10^6 dalton class had a density of 1.707 g/ml. The two largest species had a density of 1.702 g/ml. Two other strains, S. sonnei II and S. dysenteriae 60, also contained circular deoxyribonucleic acid.
Additional Information
© 1969 American Society for Microbiology. Received for publication 18 August 1969 This research was supported by Public Health Service grant GM 06967 from the National Institute of General Medical Sciences, by a grant for postdoctoral research from Merck and Co. (M.G.R.), by Public Health Service career award K3 GM 14899 (R.C.W.), and by a predoctoral fellowship from Public Health Service training grant 5 Ti GM 1234 (C.N.G.) The expert assistance of Dymetria Rush is gratefully acknowledged. ADDENDUM IN PROOF: Circular DNA has also been isolated from Shigella by H.S. Jansz, J. Zandberg, J.H. vander Pol, and E.F.J. van Bruggen (Eur. J. Biochem. 9:156, 1969).Attached Files
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Additional details
- PMCID
- PMC250161
- Eprint ID
- 5795
- Resolver ID
- CaltechAUTHORS:RUSjbact69
- GM 06967
- NIH
- Merck and Company
- K3 GM 14899
- NIH
- 5 Ti GM 1234
- NIH Predoctoral Fellowship
- Created
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2006-11-02Created from EPrint's datestamp field
- Updated
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2019-10-02Created from EPrint's last_modified field