Published March 1977
| Published
Journal Article
Open
Lac repressor purification without inactivation of DNA binding activity
Abstract
A procedure has been developed which eliminates the commonly observed inactivation of the DNA binding activity of the lac repressor during purification. The operator binding activity of the repressor obtained by this method is 100 ±10%. The repressor can be stored frozen indefinitely without losing its affinity for DNA.
Additional Information
© 1977 Oxford University Press. Received November 29, 1976. This work is contribution No. 5453 from the Norman W. Church Laboratory of Chemical Biology, California Institute of Technology. This work has been carried out with the support of the National Institutes of Health grants GM-12121 (RED) and HD-04420 (ADR), National Science Foundation grants PCM75-05586 (RED) and BMS75-14784 (ADR), and the Hyatt Corporation Research Fund (ADR).Attached Files
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Additional details
- PMCID
- PMC342462
- Eprint ID
- 1927
- Resolver ID
- CaltechAUTHORS:ROSnar77
- GM-12121
- NIH
- HD-04420
- NIH
- PCM75-05586
- NSF
- BMS75-14784
- NSF
- Hyatt Corporation Research Fund
- Created
-
2006-02-27Created from EPrint's datestamp field
- Updated
-
2021-11-08Created from EPrint's last_modified field
- Other Numbering System Name
- Norman W. Church Laboratory of Chemical Biology
- Other Numbering System Identifier
- 5453