A quantitative assay for transformation of bone marrow cells by Abelson murine leukemia virus
- Creators
- Rosenberg, Naomi
-
Baltimore, David
Abstract
A quantitative Abelson murine leukemia virus (A-MuLV) lymphoid cell transformation assay has been developed using a semisolid agarose culture system. Under these conditions lymphoid cell transformation was shown to vary linearly with the dose of A-MuLV used. The susceptibility of bone marrow cells from different strains of mice to A-MuLV-induced transformation can be estimated using the agarose assay. Strains with bone marrow cells of high, medium, and low susceptibility to A-MuLV can be identified. The assay has been used to study the susceptibility of cells from lymphoid organs of fetal and adult mice to A-MuLV. Cell suspensions from fetal liver, adult bone marrow, and adult spleen are susceptible to A-MuLV, while thymocytes are resistant to A-MuLV-induced transformation. Bovine serum albumin gradient fractionation of bone marrow cells before infection with A-MuLV demonstrates that the majority of A-MuLV-sensitive cells are recovered in a broad band partially overlapping the majority of the nucleated cells. The agarose assay system allows study of A-MuLV-lymphoid cell interaction at the level of single cell-single virus particle interaction.
Additional Information
© 1976 by Rockefeller University Press. RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Received for publication 9 February 1976. We are grateful to Dr. P.C. Kung for his advice on the use of bovine serum albumin gradients. Supported by grant no. VC-4G from the American Cancer Society, a contract from the Virus Cancer Program, and a grant no. CA-14051 from the National Cancer Institute. [N.R. was a] Postdoctoral fellow of the Massachusetts Division of the American Cancer Society. [D.B. was an] American Cancer Society Professor of Microbiology.Attached Files
Published - ROSjem76.pdf
Files
| Name | Size | Download all |
|---|---|---|
|
md5:19ab2c7250d244750bd2032d9217faed
|
1.4 MB | Preview Download |
Additional details
- PMCID
- PMC2190217
- Eprint ID
- 10677
- Resolver ID
- CaltechAUTHORS:ROSjem76
- American Cancer Society
- VC-4G
- NIH
- CA-14051
- American Cancer Society, Massachusetts Division
- Created
-
2008-06-03Created from EPrint's datestamp field
- Updated
-
2021-11-08Created from EPrint's last_modified field