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Published March 19, 1996 | Published
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Genetic evidence that the RAG1 protein directly participates in V(D)J recombination through substrate recognition

Roman, Christopher A. J.
Baltimore, David ORCID icon
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Abstract

RAG1 protein is essential for the activation of V(D)J recombination in developing lymphocytes (V, variable; D, diversity; J, joining). However, it has not been determined whether its role involves substrate recognition and catalysis. A single amino acid substitution mutation in the RAG1 gene has now been identified that renders its activity sensitive to the sequence of the coding region abutting the heptamer site in the recombination signal sequence. These results strongly imply that RAG1 interacts directly with DNA.

Additional Information

© 1996 by the National Academy of Sciences. Contributed by David Baltimore, October 18, 1995. We especially acknowledge the contribution of Dr. Patricia Cortes for an early version of the recombination PCR assay. We would like to thank Drs. Martin Gellert and Moshe Sadofsky for sharing information prior to publication. We thank Drs. Eugenia Spanopoulou and P. Cortes for immunodetection reagents, technical advice, and discussions. We thank Sara Cherry for critical evaluation of the manuscript; Ben Chen and Dr. Xiaolu Yang for valuable suggestions; and Drs. Dina Alexandropoulos, Joshy Jacob, and members of the Baltimore laboratory for discussions. We are also grateful to Pam Svec for excellent technical assistance. C.A.J.R. was supported by the Damon Runyon-Walter Winchell Cancer Research Fund and by the American Cancer Society. D.B. is an American Cancer Society Research Professor. This work was supported by National Institutes of Health Grant CA51462-08 to D.B. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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August 22, 2023
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October 13, 2023