Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome

Creators: Racaniello, Vincent R.; Baltimore, David

Abstract

The complete 7410 nucleotide sequence of the poliovirus type I genome was obtained from cloned cDNA. Double-stranded poliovirus cDNA was synthesized and inserted into the Pst I site of plasmid pBR322, and three clones were derived that together provided DNA copies of the entire poliovirus genome. Two of the clones contained inserts of 2.5 and 6.5 kilobases and represented all but the 5' 115 bases of poliovirus RNA. A third clone was generated from primer-extended DNA and contained sequences from the 5' end of the viral RNA. An open reading frame that was identified in the nucleotide sequence starting 743 bases from the 5' end of the RNA and extending to a termination codon 71 bases from the 3' end contained known poliovirus polypeptide sequence.

Additional Information

© 1981 by the National Academy of Sciences. Contributed by David Baltimore, May 11, 1981. The assistance of Mr. Robert Sege and Mr. Arthur Lee in computer analysis of our sequence and the technical assistance of Mr. Philip Hollingshead are appreciated. This work was supported by Grant AI-08388 from the National Institutes of Allergy and Infectious Diseases and Grant CA-14051 from the National Cancer Institute (core grant to S.E. Luria). V.R.R. is supported by a postdoctoral fellowship from the National Institutes of Allergy and Infectious Diseases. D.B. is an American Cancer Society Research Professor. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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