Published December 1, 1991
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Separation of Methanotrophic Bacteria by Using Percoll and Its Application to Isolation of Mixed and Pure Cultures
Abstract
Type I and II methanotrophic bacteria can be cleanly separated by using Percoll buoyant density gradients. This simple and inexpensive method can be used to screen new isolates rapidly for preliminary classification. In addition, Percoll gradients can also be used to enhance standard enrichment techniques for methanotrophs from natural water samples.
Additional Information
Copyright © 1991, American Society for Microbiology. Received 19 April 1991/Accepted 5 October 1991 We acknowledge those who contributed to this work in one way or another: Steve Konings, a partner in the Menomonee River work; Marilyn Schaller for assistance with TEM; and Barb Wimpee for some of the illustrations. This work was supported in part by the Center for Great Lakes Studies and in part by grant 2022-1RP-79 from the NASA Interdisciplinary Research Program in Earth Sciences. Contribution no. 355 from the Center for Great Lakes Studies.Files
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