Characterization of Staphylococcus aureus Enterotoxin L
Abstract
Staphylococcus aureus causes a wide variety of diseases. Major virulence factors of this organism include enterotoxins (SEs) that cause both food poisoning and toxic shock syndrome. Recently, a novel SE, tentatively designated SEL, was identified in a pathogenicity island from a bovine mastitis isolate. The toxin had a molecular weight of 26,000 and an isoelectric point of 8.5. Recombinant SEL shared many biological activities with SEs, including superantigenicity, pyrogenicity, enhancement of endotoxin shock, and lethality in rabbits when administered in subcutaneous miniosmotic pumps, but the protein lacked emetic activity. T cells bearing the T-cell receptor ß chain variable regions 5.1, 5.2, 6.7, 16, and 22 were significantly stimulated by recombinant SEL.
Additional Information
Copyright © 2003, American Society for Microbiology. Received 26 November 2002/Returned for modification 24 January 2003/Accepted 12 February 2003. This work was supported by research grants from the NIH (AI22159, AI2840, RR15587, HL37260, AR41256, and HL36577), the USDA NRI (99-35201-8581), and the Idaho Agricultural and Experiment Station. Primate experiments were conducted at the University of Washington Regional Primate Facility (NIH grant RR00166) with technical assistance from Debra Glanister and Ed Novak. P.M.O. was supported by a National Science Foundation fellowship and a USPHS/NIAID training grant (5 T32 AI07421). Tim Leonard is gratefully acknowledged for help in generating figures.Files
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Additional details
- Eprint ID
- 8829
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- CaltechAUTHORS:ORWiai03
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2007-09-20Created from EPrint's datestamp field
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2021-11-08Created from EPrint's last_modified field