Published January 1, 1973
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Journal Article
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Stability of Cytoplasmic Messenger RNA in HeLa Cells
- Creators
- Murphy, William
- Attardi, Giuseppe
Chicago
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Abstract
The metabolic stability of pulse-labeled or long-term labeled messenger RNA (mRNA) from cytoplasmic free polysomes was measured in HeLa cells, with chase conditions that do not involve inhibitors of RNA synthesis, and chromatography on benzoylated-DEAE-cellulose or poly(T)-cellulose for isolation of mRNA. For these studies, a new chase technique was developed that allows analysis of the stability of mRNA labeled during a short [3H]uridine pulse. Pulse-labeled and long-term labeled mRNA were found to decay with an estimated average half-life of about 2 and 3 days, respectively, much longer than hitherto assumed.
Additional Information
Copyright © 1973 by the National Academy of Sciences Communicated by Norman Davidson, October 24, 1972 These investigations were supported by a research grant from the U.S. Public Health Service (GM-11726) and by a National Science Foundation Predoctoral Fellowship to W. M. The help of Mrs. L. Wenzel and Miss G. Engel and the excellent technical assistance of Mrs. B. Keeley are gratefully acknowledged. The poly(T)-cellulose used here was a generous gift of Dr. J. Kates.Files
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- Resolver ID
- CaltechAUTHORS:MURpnas73
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