Monoclonal Antibody Cross-Reactions between Drosophila and Human Brain

Creators: Miller, Carol A.; Benzer, Seymour

Abstract

A panel of 146 monoclonal antibodies (MAbs), obtained with Drosophila melanogaster tissue as primary immunogen, was tested for cross-reactivity with the human central nervous system. Sites examined included spinal cord, cerebellum, hippocampus, and optic nerve. Nonnervous tissues tested were liver and lymph node. Approximately half of the antibodies reacted with one or more sites in the human central nervous system, identifying regional, cell class, and subcellular antigens. Some recognized neuronal, glial, or axonal subsets. Immunoblot analysis revealed that some antibodies reacted with similar antigen patterns in both species.

Additional Information

© 1983 by the National Academy of Sciences Contributed by Seymour Benzer, August 24, 1983 We thank Celia Williams, Tulan Do, Mimi Gee, Robert Cowan, Devra Ellert, and Marika Szalay for assistance with antibody production and screening of sections and Eulah Holland for secretarial assistance. Stephen L. Zipursky and Tadmiri Venkatesh provided helpful advice on techniques. This work was supported by grants to C.A. M. from the Barbara Vanderbilt Peck Fund for Neurological Research of the Amyotrophic Lateral Sclerosis Society of America, the Muscular Dystrophy Association, the Heredity Disease Foundation, Grant 5R01 NS15811 from the National Institutes of Health, and National Science Foundation Grant PCM-7911771 to S.B. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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