Direct Observation of the Torsional Dynamics of DNA and RNA by Picosecond Spectroscopy

Abstract

Picosecond time-dependent fluorescence depolarization techniques have been used to monitor the reorientation of ethidium bromide intercalated in DNA and RNA. The fluorescence polarization anisotropy reveals a nonexponential, exp (-at^(1/2)), torsional relaxation of the DNA double helix and provides an accurate value for its torsional rigidity, C=1.3± 0.2 x 10^-19 erg cm. Furthermore, from accurate measurements of the limiting anisotropy at zero time, we conclude that there is an additional fast (<10 psec) internal motion that depends on the viscosity of the medium. Denatured DNA is considerably more flexible than the intact double helix, thus demonstrating the influence of secondary structure on internal motions.

Files

Additional details