Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published May 1971 | Published
Journal Article Open

Genetic Expression in Heterozygous Replicative Form Molecules of {varphi}X174

Abstract

Heterozygous replicative form molecules of bacteriophage {varphi}X174 deoxyribonucleic acid (DNA) have been constructed in vitro. These are composed of viral strands extracted from purified preparations of phage bearing ts mutations and complementary strands of either half length or full length synthesized with purified DNA polymerase, in vitro, on DNA from am3 phage. In infections with such heterozygous DNA, involving mutations in each of four different cistrons, phage with the genotype of the complementary strand comprised 1 to 20% of the total phage produced by a spheroplast population. From single-burst analysis of the progeny from DNA heterozygous in one cistron (B), it appears that those phage with the genotype of the complementary strand arise as major components in a small proportion of the infected cells rather than comprising a minor component in most cells. The implications of such a pattern of expression are discussed with respect to mechanisms of phage DNA synthesis.

Additional Information

© 1971 American Society for Microbiology. Received for publication 4 January 1971 This research was supported by Public Health Service grant GM 13554 from the National Institute of General Medical Sciences. One of us (V.M.) wishes to thank Dan Ray for the use of the facilities of UCLA for the completion of some of these experiments.

Attached Files

Published - MERjvir71.pdf

Files

MERjvir71.pdf
Files (1.4 MB)
Name Size Download all
md5:d1fc800e621ee94405a27ab20df093db
1.4 MB Preview Download

Additional details

Created:
August 22, 2023
Modified:
October 23, 2023