Perturbed angular correlation study of a haptenic molecule
Abstract
The angular correlation of the 173-247 keV gamma-ray cascade after the electron-capture decay of (111)In is strongly perturbed when the I-p-nitrophenylethylenediaminetetraacetate chelate of (111)In(3+) is added to a solution containing rabbit antibody to dinitrophenyl groups. The radioactive chelate can be displaced by the addition of dinitrophenyllysine or unlabeled chelate. The average association constant between the antibody and the labeled chelate has been estimated from perturbed angular correlation measurements; this value is compared to the results of equilibrium dialysis. These experiments provide good evidence that information concerning macromolecular behavior can be obtained from perturbed angular correlation experiments that use chemically specific labels.
Additional Information
Copyright © 1972 by the National Academy of Sciences. Contributed by John D. Baldeschwieler, October 5, 1972. We thank Dr. G. Rosenquist, Dr. J. H. Freed, and Prof. H. O. McDevitt for their advice and help. This work was supported by the National Institutes of Health (GM-14752), the National Science Foundation (GP4924), and the Center for Materials Research, Stanford University. C. F. M. was supported by a National Science Foundation Graduate Fellowship (1968-1972).Files
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Additional details
- Eprint ID
- 1167
- Resolver ID
- CaltechAUTHORS:MEApnas72
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2005-12-30Created from EPrint's datestamp field
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2019-10-02Created from EPrint's last_modified field