Dynamic Analyses of Drosophila Gastrulation Provide Insights into Collective Cell Migration
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1.
California Institute of Technology
Abstract
The concerted movement of cells from different germ layers contributes to morphogenesis during early embryonic development. Using an optimized imaging approach and quantitative methods, we analyzed the trajectories of hundreds of ectodermal cells and internalized mesodermal cells within Drosophila embryos over 2 hours during gastrulation. We found a high level of cellular organization, with mesoderm cell movements correlating with some but not all ectoderm movements. During migration, the mesoderm population underwent two ordered waves of cell division and synchronous cell intercalation, and cells at the leading edge stably maintained position. Fibroblast growth factor (FGF) signaling guides mesodermal cell migration; however, we found some directed dorsal migration in an FGF receptor mutant, which suggests that additional signals are involved. Thus, decomposing complex cellular movements can provide detailed insights into collective cell migration.
Additional Information
© 2008 American Association for the Advancement of Science. 16 May 2008; accepted 12 November 2008. We thank M. Levine and E. Meyerowiz for comments on the manuscript, M. Liebling for advice on Imaris and Matlab, and the Caltech Biological Imaging Center for sharing equipment. This work was supported by grants to A.S. from NIH (R01 GM078542), the Searle Scholars Program, and the March of Dimes (Basil O'Conner Starter Scholar Award, 5-FY06-12), and grants to S.F. from the Caltech Beckman Institute and NIH (Center for Excellence in Genomic Science grant P50 HG004071). Supporting Online Material: http://www.sciencemag.org/cgi/content/full/322/5907/1546/DC1 Materials and Methods; Figs. S1 to S7; Movie S1 to S11.Attached Files
Accepted Version - nihms163222.pdf
Supplemental Material - MCMsci08movieS1.mov
Supplemental Material - MCMsci08movieS10.mov
Supplemental Material - MCMsci08movieS11.mov
Supplemental Material - MCMsci08movieS2.mov
Supplemental Material - MCMsci08movieS3.mov
Supplemental Material - MCMsci08movieS4.mov
Supplemental Material - MCMsci08movieS5.mov
Supplemental Material - MCMsci08movieS6.mov
Supplemental Material - MCMsci08movieS7.mov
Supplemental Material - MCMsci08movieS8.mov
Supplemental Material - MCMsci08movieS9.mov
Supplemental Material - MCMsci08supp.pdf
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Additional details
- PMCID
- PMC2801059
- Eprint ID
- 12529
- Resolver ID
- CaltechAUTHORS:MCMsci08
- NIH
- R01 GM078542
- Searle Scholars Program
- March of Dimes
- 5-FY06-12
- Caltech Beckman Institute
- NIH
- P50 HG004071
- Created
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2008-12-16Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field