Published December 31, 2006
| Published
Journal Article
Open
Fluorescence Near-Field Microscopy of DNA at Sub-10 nm Resolution
Chicago
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Abstract
We demonstrate apertureless near-field microscopy of single molecules at sub-10 nm resolution. With a novel phase filter, near-field images of single organic fluorophores were obtained with ~sixfold improvement in the signal-to-noise ratio. The improvement allowed pairs of molecules separated by ~15 nm to be reliably and repeatedly resolved, thus demonstrating the first true Rayleigh resolution test for near-field images of single molecules. The potential of this technique for biological applications was demonstrated with an experiment that measured the helical rise of A-form DNA.
Additional Information
© 2006 The American Physical Society (Received 21 October 2005; published 27 December 2006) The authors thank G. Lessard for contributions to the development of initial data acquisition and data analysis algorithms. This work was supported by Pharmagenomix Inc. and the NIH Director's Pioneer program.Attached Files
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Additional details
- Eprint ID
- 6859
- Resolver ID
- CaltechAUTHORS:MAZprl06
- Pharmagenomix Inc.
- NIH
- Created
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2006-12-29Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field