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Published December 2003 | public
Journal Article

Non-canonical amino acids in protein engineering

Abstract

Methods for engineering proteins that contain non-canonical amino acids have advanced rapidly in the past few years. Novel amino acids can be introduced into recombinant proteins in either a residue-specific or site-specific fashion. The methods are complementary: residue-specific incorporation allows engineering of the overall physical and chemical behavior of proteins and protein-like macromolecules, whereas site-specific methods allow mechanistic questions to be probed in atomistic detail. Challenges remain in the engineering of the translational apparatus and in the design of schemes that can be used to encode both canonical and non-canonical amino acids.

Additional Information

© 2003 Elsevier. Work on non-canonical amino acids at Caltech has been supported by grants from the National Science Foundation, the National Institutes of Health and the US Army Research Office.

Additional details

Created:
August 22, 2023
Modified:
October 19, 2023