Published September 3, 1996
| Published
Journal Article
Open
Design of artificial sequence-specific DNA bending ligands
- Creators
- Liberles, David A.
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Dervan, Peter B.
Abstract
Proteins that bend DNA are important regulators of biological processes. Sequence-specific DNA bending ligands have been designed that bind two noncontiguous sites in the major groove and induce a bend in the DNA. An oligonucleotide containing pyrimidine segments separated by a central variable linker domain simultaneously binds by triple helix formation two 15-bp purine tracts separated by 10 hp. Bend angles of 61 degrees, 50 degrees, and 38 degrees directed towards the minor groove were quantitated by phasing analysis for linkers of four, five, and six T residues, respectively. The design and synthesis of nonnatural architectural factors may provide a new class of reagents for use in biology and human medicine.
Additional Information
© 1996 by the National Academy of Sciences. Contributed by Peter B. Dervan, April 15, 1996. We are grateful for support from the National Institutes of Health (Grant GM 51747). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.Attached Files
Published - LIBpnas96.pdf
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LIBpnas96.pdf
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Additional details
- PMCID
- PMC38459
- Eprint ID
- 1571
- Resolver ID
- CaltechAUTHORS:LIBpnas96
- NIH
- GM-51747
- Created
-
2006-01-30Created from EPrint's datestamp field
- Updated
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2019-11-26Created from EPrint's last_modified field