Published July 1974
| Published
Journal Article
Open
A cleavage map of bacteriophage Phi-X174 genome
- Creators
- Lee, Amy Shiu
- Sinsheimer, Robert L.
Abstract
Restriction endonucleases isolated from Hemophilus influenzae, Hemophilus parainfluenzae, and Hemophilus aegyptius were used to cleave phi-X174 replicative form DNA into three sets of specific DNA fragments. The order of these fragments in the phi-X replicative form molecule was determined by (1) analysis of partial digest products, (2) analysis of overlapping sets of fragments produced by two different restrictive enzymes. On the basis of these results, a detailed physical map of the phi-X174 genome has been constructed with respect to the cleavage sites of all three enzymes.
Additional Information
© 1974 by the National Academy of Sciences. Contributed by Robert L. Sinsheimer, April 10, 1974. We thank Dr. C. Hutchinson, III for sending us the H. aegyptius strain, Dr. P.H. Johnson for the generous supply of [32P]phi-X RF, and Ms. Carole Latham for excellent technical assistance. This investigation was supported by the McCallum Fund of the Division of Biology of the California Institute of Technology and by USPHS Grant GM13554.Attached Files
Published - LEEpnas74.pdf
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LEEpnas74.pdf
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Additional details
- PMCID
- PMC388576
- Eprint ID
- 7728
- Resolver ID
- CaltechAUTHORS:LEEpnas74
- Caltech McCallum Fund
- GM 13554
- U.S. Public Health Service (USPHS)
- Created
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2007-07-30Created from EPrint's datestamp field
- Updated
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2019-10-02Created from EPrint's last_modified field