Published June 1, 1974
| public
Journal Article
Open
Actin antibody: The specific visualization of actin filaments in non-muscle cells
- Creators
- Lazarides, Elias
- Weber, Klaus
Abstract
Actin purified from mouse fibroblasts by sodium dodecyl sulfate gel electrophoresis was used as antigen to obtain an antibody in rabbits. The elicited antibody was shown to be specific for actin as judged by immunodiffusion and complement fixation against partially purified mouse fibroblast actin and highly purified chicken muscle actin. The antibody was used in indirect immunofluorescence to demonstrate by fluorescence light microscopy the distribution and pattern of actin-containing filaments in a variety of cell types. Actin filaments were shown to span the cell length or to concentrate in "focal points" in patterns characteristic for each individual cell.
Additional Information
© 1974 by the National Academy of Sciences. Communicated by J. D. Watson, March 11, 1974. We thank Dr. F. Miller at Stonybrook University for the use of his animal facilities and his help in the preparation of the antibodies. We thank Dr. R. Pollack for the use of his laboratory; Drs. R. Goldman and C.-M. Chang and Art Vogel for their help with the fluorescent microscopy; and Drs. M. Osborn, C.W. Anderson, R. Goldman, and R. Pollack for their comments on the manuscript. We also thank Dr. Susan Lowey for a generous gift of purified chicken muscle actin. This investigation was supported by the National Institutes of Health (Research Grant CA-13106 from the National Cancer Institute).Files
LAZpnas74a.pdf
Files
(2.0 MB)
| Name | Size | Download all |
|---|---|---|
|
md5:515f2f253dde58b12f51c2de1861d832
|
2.0 MB | Preview Download |
Additional details
- Eprint ID
- 7477
- Resolver ID
- CaltechAUTHORS:LAZpnas74a
- Created
-
2007-02-23Created from EPrint's datestamp field
- Updated
-
2021-11-08Created from EPrint's last_modified field