Cloning and analysis of reverse transcript P160 genomes of Abelson murine leukemia virus
Abstract
Circular duplex reverse transcripts of the genome of a strain of Abelson murine leukemia virus that encodes a 160,000-molecular-weight protein were isolated, cleaved with HindIII restriction endonuclease, and cloned into the unique HindIII site of lambda phage Charon 21A. Recombinant phage clones, some of which were infectious in transfection assays, were found to contain a 789-base-pair region specific for Abelson murine leukemia virus; this region is not found in other strains of this virus. The extra sequence was localized by restriction endonuclease and electron microscopic heteroduplex analysis. Sequence analysis showed no homology at the ends of the extra sequence, implying that it was deleted by an event that did not utilize sequence homology. The sequence of this unique region has an open reading frame through its entirety.
Additional Information
Copyright © 1983 by the American Society for Microbiology. Received 27 September 1982. Accepted 22 November 1982. We thank Chris Riser for advice on the use of the electron microscope. This work was supported by Public Health Service grants CA-26717 (to D.B.), GM-21121 (to S.A.L.), and CA-14501 (core grant to S. E. Luria) from the National Institutes of Health. S.P.G. was a postdoctoral fellow of the Jane Coffin Childs Memorial Fund for Medical Research, as is J.Y.-J.W. D.B. is an American Cancer Society Research Professor.Files
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Additional details
- Eprint ID
- 3620
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- CaltechAUTHORS:LATjvir83
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2006-06-21Created from EPrint's datestamp field
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2019-10-02Created from EPrint's last_modified field