Genomic organization of the mouse T-cell receptor β-chain gene family

Creators: Lai, Eric; Barth, Richard K.; Hood, Leroy

Abstract

We have combined three different methods, deletion mapping of T-cell lines, field-inversion gel electrophoresis, and the restriction mapping of a cosmid clone, to construct a physical map of the murine T-cell receptor β-chain gene family. We have mapped 19 variable (Vβ) gene segments and the two clusters of diversity (Dβ) and joining (Jβ) gene segments and constant (Cβ) genes. These members of the β-chain gene family span ~450 kilobases of DNA, excluding one potential gap in the DNA fragment alignments.

Additional Information

© 1987 by the National Academy of Sciences. Contributed by Leroy Hood, February 17, 1987. We thank Drs. Joan Kobori, Nilabh Shastri, Mark Davis, Edward Palmer, and Paul Singer for providing Va, DNA probes, Frank Fitch, Esther Hayes, and Miriam Lieberman for T-cell lines, and Nilabh Shastri for the BO4H.9.1 T-cell hybridoma. We also thank Catherine Speiser for excellent technical assistance, Drs. Patrick Concannon, Ulf Landegren, Brian Popko, and Lloyd Smith for critical review of the manuscript, and Bertha Jones and Stephanie Canada for preparation of the manuscript. E.L. is a Leukemia Society of America Fellow and R.K.B. is supported by the Cancer Research Institute/Eleanor Naylor Dana Fellowship. This work was supported by National Institutes of Health Grant AI 17565 to L.H. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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