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Published April 24, 2001 | Published + Erratum
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Activation-dependent changes in receptor distribution and dendritic morphology in hippocampal neurons expressing P2X(2)-green fluorescent protein receptors

Khakh, Baljit S.
Smith, W. Bryan
Chiu, Chi-Sung
Ju, Donghong
Davidson, Norman
Lester, Henry A. ORCID icon
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Abstract

ATP-gated P2X(2) receptors are widely expressed in neurons, but the cellular effects of receptor activation are unclear. We engineered functional green fluorescent protein (GFP)-tagged P2X(2) receptors and expressed them in embryonic hippocampal neurons, and report an approach to determining functional and total receptor pool sizes in living cells. ATP application to dendrites caused receptor redistribution and the formation of varicose hot spots of higher P2X(2)-GFP receptor density. Redistribution in dendrites was accompanied by an activation-dependent enhancement of the ATP-evoked current. Substate-specific mutant T18A P2X(2)-GFP receptors showed no redistribution or activation-dependent enhancement of the ATP-evoked current. Thus fluorescent P2X(2)-GFP receptors function normally, can be quantified, and reveal the dynamics of P2X(2) receptor distribution on the seconds time scale.

Additional Information

© 2001 by the National Academy of Sciences. Contributed by Norman Davidson, February 21, 2001. We thank H. Li for preparation of Xenopus oocytes and E. Schuman for advice and use of the confocal microscope. This work was supported by a Wellcome Trust (U.K.) Prize Fellowship (to B.S.K.), by a Roche Bioscience Award, and by the National Institutes of Health (NS-11756, MH 49176). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

Errata

For the article "Activation-dependent changes in receptor distribution and dendritic morphology in hippocampal neurons expressing P2X2-green fluorescent protein receptors" by Baljit S. Khakh, W. Bryan Smith, Chi-Sung Chiu, Donghong Ju, Norman Davidson, and Henry A. Lester, which appeared in number 9, April 24, 2001, of Proc. Natl. Acad. Sci. USA (98, 5288–5293; First Published April 10, 2001; 10.1073/pnas.081089198), due to a printer's error, Baljit Khakh's e-mail was incorrectly listed as bsk@mrc-lmb.com.ac.uk. It should be ku.ca.mac.bml-crm@ksb.

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Erratum - KHApnas01errata.pdf

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