Published July 9, 1996
| Published
Journal Article
Open
Binding site size limit of the 2:1 pyrrole-imidazole polyamide-DNA motif
Abstract
Polyamides containing N-methylimidazole (Im) and N-methylpyrrole (Py) amino acids can be combined in antiparallel side-by-side dimeric complexes for sequence-specific recognition in the minor groove of DNA. Six polyamides containing three to eight rings bind DNA sites 5-10 bp in length, respectively. Quantitative DNase I footprint titration experiments demonstrate that affinity maximizes and is similar at ring sizes of five, six, and seven. Sequence specificity decreases as the length of the polyamides increases beyond five rings. These results provide useful guidelines for the design of new polyamides that bind longer DNA sites with enhanced affinity and specificity.
Additional Information
© 1996 by the National Academy of Sciences. Contributed by Peter B. Dervan, March 26, 1996. We are grateful to the National Institutes of Health for grant support (GM-27681) and a National Research Service Award to J.J.K., and to the Howard Hughes Medical Institute for a predoctoral fellowship to E.E.B. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.Attached Files
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Additional details
- PMCID
- PMC38920
- Eprint ID
- 1566
- Resolver ID
- CaltechAUTHORS:KELpnas96
- NIH
- GM-27681
- NIH Predoctoral Fellowship
- Howard Hughes Medical Institute (HHMI)
- Created
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2006-01-30Created from EPrint's datestamp field
- Updated
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2019-11-26Created from EPrint's last_modified field