Published July 1, 1988
| Published
Journal Article
Open
Nonenzymatic sequence-specific methyl transfer to single-stranded DNA
- Creators
- Iverson, Brent L.
-
Dervan, Peter B.
Abstract
2'-Deoxyuridine 5'-triphosphate with a methylthioether moiety at the 5 position can be incorporated into a primer-template DNA complex with Klenow enzyme. Activation with CNBr at 25 degrees C, pH 5.5, followed by treatment with piperidine produces sequence-specific cleavage on the template DNA predominantly at a single guanine position. The mechanism involves methyl-group transfer from sulfur on the modified deoxyuridine of the extended primer to N-7 of guanine on the template DNA. This raises the possibility for the design and synthesis of a nonenzymatic class of sequence-specific methyl-transferases for DNA.
Additional Information
© 1988 by the National Academy of Sciences. Contributed by Peter B. Dervan, February 24, 1988. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.Attached Files
Published - IVEpnas88.pdf
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IVEpnas88.pdf
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Additional details
- PMCID
- PMC280485
- Eprint ID
- 1557
- Resolver ID
- CaltechAUTHORS:IVEpnas98
- Created
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2006-01-30Created from EPrint's datestamp field
- Updated
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2019-11-26Created from EPrint's last_modified field