Nitrogen distribution by globin
- Creators
- Hunter, Andrew
- Borsook, Henry
Abstract
This and other experiences with the tryptophane method of Fürth and Nobel led us to doubt seriously the reliability of quantitative data obtained by its application. When, therefore, just as we completed our work with it, Folin and Looney (6) described another and apparently better method of determination, a method based upon a different color reaction and capable moreover of convenient combination with a quantitative procedure for tyrosine, it seemed to us worth while to review the problem again. With the aid of this newer method we have now determined the tryptophane and tyrosine content of two series of globin preparations, and have, we believe, settled fairly decisively the proportion of these amino-acids yielded by the pure protein. We have also taken occasion to determine by the method of Van Slyke the general distribution of nitrogen in the globin molecule.
Additional Information
© 1923 American Society of Biological Chemists. (Received for publication, July 2, 1923.)Attached Files
Published - HUNjbc23.pdf
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Additional details
- Eprint ID
- 9373
- Resolver ID
- CaltechAUTHORS:HUNjbc23
- Created
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2007-12-16Created from EPrint's datestamp field
- Updated
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2019-10-02Created from EPrint's last_modified field