Published October 1966
| Published
Journal Article
Open
Isolation of metaphase chromosomes from HeLa cells
- Creators
- Huberman, Joel A.
- Attardi, Giuseppe
Chicago
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Abstract
The authors have developed a method for large-scale isolation of metaphase chromosomes from HeLa cells. The distinguishing feature of this method is the use of a pH sufficiently low (about 3) to stabilize the chromosomes against mechanical damage. Many milligrams of fairly pure, morphologically intact chromosomes can be isolated in 8 hr or less of total working time. The isolated chromosomes contain about 2.0 mg of acid-soluble protein, 2.7 mg of acid-insoluble protein and 0.66 mg of RNA for each milligram of DNA. The RNA bound to the isolated chromosomes consists mainly of ribosomal RNA, but there is also a significant amount of 45S RNA.
Additional Information
© 1966 by Rockefeller University Press. Received for publication 4 April 1966. This work was supported by United States Public Health Service grants GM-11726 and 5-F1-GM-21,622. The authors gratefully acknowledge the help of Mr. John Elberfeld in part of this work and the valuable technical assistance of Mrs. Benneta Keeley and Mrs. LaVerne Wenzel.Attached Files
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Additional details
- PMCID
- PMC2107040
- Eprint ID
- 8903
- Resolver ID
- CaltechAUTHORS:HUBjcb66
- NIH
- GM-11726
- NIH Postdoctoral Fellowship
- 5-F1-GM-21,622
- Created
-
2007-09-25Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field