Identification of a component of Drosophila polar granules
Abstract
Information necessary for the formation of pole cells, precursors of the germ line, is provided maternally and localized to the posterior pole of the Drosophila egg. The maternal origin and posterior localization of polar granules suggest that they may be associated with pole cell determinants. We have generated an antibody (Mab46F11) against polar granules. In oocytes and early embryos, the Mab46F11 antigen is sharply localized to the posterior embryonic pole. In pole cells, it becomes associated with nuclear bodies within, and nuage around, the nucleus. Immunoreactivity remains associated with cells of the germ line throughout the life cycle of both males and females. This antibody recognizes a 72-74 X 10^(3) Mr protein and is useful both as a pole lineage marker and in biochemical studies of polar granules.
Additional Information
© 1988 by Company of Biologists. (Accepted 11 April 1988) We would like to thank T. Schüpbach and E. Wieschaus for sending us the vasaPD23 stock, and R. Lehmann and C. Nusslein-Volhard for sending us the pumillio680 stock. We also thank T. Kornberg for use of his fly cage facility. We especially thank K. Logan for help analysing the data concerning pole cell number, E. Bier, K. Logan, K. Neugebauer, and T. Schwarz for their valuable comments on the manuscript and Ms L. Schulte for help in preparing the manuscript. This work was supported by NTH and HHMI grants to L.Y.J. and Y.N.J. B.H. was supported by an NLH predoctoral training gTant. L.Y.J. and Y.N.J. are HHMI investigators.Attached Files
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Additional details
- Eprint ID
- 11587
- Resolver ID
- CaltechAUTHORS:HAYdev88
- NIH
- Howard Hughes Medical Institute (HHMI)
- Created
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2008-09-08Created from EPrint's datestamp field
- Updated
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2020-07-02Created from EPrint's last_modified field