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Published February 1978 | Published
Journal Article Open

Vesicular stomatitis virus glycoprotein is necessary for H-2-restricted lysis of infected cells by cytotoxic T lymphocytes

Abstract

Vesicular stomatitis virus (VSV) elicited cytotoxic thymus-derived lymphocytes (CTLs) in mice of the BALB/c and three congenic strains (BALB.b, BALB.k, BALB.HTG). CTL lysis of VSV-infected fibroblasts from the four strains was restricted by the target cells' major histocompatibility complex (H-2). Target cells were also infected with two temperature-sensitive mutants of VSV, tsM and tsG in which, respectively, the viral matrix protein and glycoprotein are not expressed at 39 degrees (restrictive temperature) on the infected cell's surface membrane. At the restrictive temperature, cells infected with wild-type VSV or tsM were lysed by CTLs, but cells infected with tsG were not. The requirement for the glycoprotein on the target cell was also evident from the ability of antisera to the glycoprotein to block completely CTL lysis of VSV-infected cells.

Additional Information

© 1978 by the National Academy of Sciences. Contributed by Herman N. Eisen, December 5, 1977. We thank E. A. Boyse, R. J. Graff, and F. Lilly for the breeders used to establish colonies of BALB/cAnN, BALB.b, BALB.k, and BALB.HTG mice. D.B. is a Research Professor of the American Cancer Society; A.H.H. is the recipient of Postdoctoral Fellowship Award 5 F32 CA05685 from the National Cancer Institute; O.N.W. is a fellow of the Helen Hay Whitney Foundation. This work was supported by Research Grant VC-41 from the American Cancer Society and by Research Grant CA-15472 and Center Grant CA-14051 from the National Cancer Institute, Department of Health, Education, and Welfare. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.

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August 22, 2023
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October 16, 2023