Published August 1, 1976
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Journal Article
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Electron microscope study of a plasmid chimera containing the replication region of the Escherichia coli F plasmid
Chicago
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Abstract
pML31, a plasmid chimera constructed to contain the replication genes of an Flac plasmid, has been studied by electron microscope methods. Heteroduplex analysis shows that the only F sequence present in pML31 is that with corrdinates 40.3-49.3F. This region has previously been identified as essential for plasmid maintenance. The sequence of pML31, which was derived originally from R6-5, carries the km gene(s) and an inverted duplication of a 1.0-kilobase sequence. On the basis of length measurements, the repeated sequence is different from IS1, IS2, IS3, and an inverted repeat associated with the km gene(s) of plasmid JR67.
Additional Information
Copyright © 1976 by the American Society for Microbiology. Received for publication 3 February 1976 This research has been supported by Public Health Service grants GM10991 from the National Institute of General Medical Sciences and AI-07194 from the National Institute of Allergy and Infectious Diseases, and National Science Foundation grant GB-29492. Mark Guyer is a Fellow of the Jane Coffin Childs Memorial Fund for Medical Research. David Figurski is a Public Health Service research fellow (1-F22-AI-01412, National Institute of Allergy and Infectious Diseases).Files
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