A fluorometric method for the estimation of tryptophan
- Creators
- Gordon, Malcolm
- Mitchell, Herschel K.
Abstract
The various colorimetric methods now employed for the estimation of tryptophan are not specific for tryptophan but give colored products with many compounds containing the indole nucleus, including indole itself. In order to facilitate studies of the enzymatic synthesis of tryptophan from indole and serine by extracts of Neurospora (1), a search for a rapid, quantitative method for the estimation of tryptophan in the presence of indole was undertaken. Tauber (2) reported that tryptophan gives a green fluorescence when treated with 70 to 72 per cent perchloric acid at room temperature. Modification of this procedure has led to a method for the rapid estimation of tryptophan, without preliminary extraction of indole, in hydrolyzed or unhydrolyzed tryptophan-containing materials.
Additional Information
© 1949 by the American Society for Biochemistry and Molecular Biology. Received for publication, May 7, 1949. This work was supported by funds from the Rockefeller Foundation and by funds from the Atomic Energy Commission, administered through contract with the Office of Naval Research, United States Navy (contract N-6-onr-244, Task Order 5). [M.G. was a] Merck Fellow of the National Research Council.Attached Files
Published - GORjbc49.pdf
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Additional details
- Eprint ID
- 11557
- Resolver ID
- CaltechAUTHORS:GORjbc49
- Rockefeller Foundation
- Atomic Energy Commission
- N-6-onr-244, Task Order 5
- Office of Naval Research
- National Research Council
- Created
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2008-09-12Created from EPrint's datestamp field
- Updated
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2019-10-03Created from EPrint's last_modified field