Published December 1973
| Published
Journal Article
Open
Nonrepetitive DNA sequence representation in sea urchin embryo messenger RNA
Abstract
Messenger RNA was prepared from developing sea urchin gastrulae by puromycin release from polyribosomes. Approximately 60% of the total mRNA radioactivity of the postnuclear supernatant was recovered and shown to be free of any other labeled RNA species such as ribosomal and nuclear RNA. The mRNA was examined by hybridization to DNA present in great excess. The mRNA hybridizes almost exclusively with nonrepetitive DNA. Almost all of the messenger RNA molecules of sea urchin gastrulae therefore consist of transcripts from nonrepetitive sequences. It appears that the structural genes expressed at this stage are typically not repeated in the genome and the mRNA does not include recognizable repetitive sequence.
Additional Information
© 1973 by the National Academy of Sciences. Contributed by Roy J. Britten, July 13, 1973. This research was supported by National Science Foundation Grant GB-33441X and by a grant from the National Institutes of Health (HD05753). R.B.G. was the recipient of an NIH Postdoctoral Fellowship GM51391. G.A.G. is a predoctoral fellow on USPH Training Grant GM 00086.Attached Files
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Additional details
- PMCID
- PMC427271
- Eprint ID
- 9565
- Resolver ID
- CaltechAUTHORS:GOLpnas73
- GB-33441X
- NSF
- HD05753
- NIH
- GM51391
- NIH Postdoctoral Fellowship
- GM 00086
- NIH Predoctoral Fellowship
- Created
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2008-02-05Created from EPrint's datestamp field
- Updated
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2019-10-03Created from EPrint's last_modified field