Biosynthesis of the actinomycin chromophore: incorporation of 3-hydroxy-4-methylanthranilic acid into actinomycins by streptomyces antibioticus
Abstract
Actinomycin synthesis by washed mycelia of Streptomyces antibioticus has been conducted in the presence of 3-hydroxy-4-methylanthranilate-(carboxyl-14C). Incorporation of this compound into actinomycins has been observed, which constitutes further evidence that 3-hydroxy-4-methylanthranilate is an intermediate in actinomycin biosynthesis. The position of the incorporated label has been determined to be within the actinomycin chromophore, and the label appears to be equally distributed between both halves of the chromophore. Incidental to these findings was the observation that the 14C-labeled actinomycins were subject to rapid reabsorption by the organism with actinomycin V taken up preferentially to actinomycin IV.
Additional Information
© 1969 American Society for Microbiology. Received for publication 12 August 1969. The authors wish to thank Steven H. Weinograd for skillful technical assistance. This investigation was supported by Public Health Service grant AI-06366 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, grant GB8083 from the National Science Foundation, and the Tufts University Cancer Committee.Attached Files
Published - GOLjbact69.pdf
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Additional details
- PMCID
- PMC250184
- Eprint ID
- 4372
- Resolver ID
- CaltechAUTHORS:GOLbact69
- NIH
- AI-06366
- NIH
- GB8083
- Tufts University
- Created
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2006-08-18Created from EPrint's datestamp field
- Updated
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2019-10-02Created from EPrint's last_modified field