Published February 1, 1987
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Journal Article
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The excised leader of human cytochrome c oxidase subunit I mRNA which contains the origin of mitochondrial DNA light-strand synthesis accumulates in mitochondria and is polyadenylated
- Creators
- Gaines, George
- Rossi, Cesare
- Attardi, Giuseppe
Abstract
We identified a polyadenylated RNA species which contains the origin of human mitochondrial DNA light-strand synthesis and the surrounding complementary sequences of the four light-strand-encoded tRNAs. This RNA (RNA 9L) is probably derived from the leader portion of RNA 6 which is excised during the formation of the mature cytochrome c oxidase subunit mRNA (RNA 9). The high degree of secondary structure of this RNA is presumably responsible for its anomalous electrophoretic behavior in denaturing polyacrylamide gels.
Additional Information
Copyright © 1987 by the American Society for Microbiology. Received 8 July 1986/Accepted 6 November 1986 These investigations were supported by grants GNI-11726 and T32GM-07616 from the National Institutes of Health Public Health Service. One of us (C.R.) was a recipient of a Gosney Fellowship and a Fellowship of the Associazione Italiana per la Ricerca sul Cancro. We thank Michael King for his generous gift of several M13 clones of human mtDNA. Anne Chomyn for the computer analysis, and Jan Hoh for help in the sequencing experiments. The technical assistance of Arger Drew is gratefully acknowledged.Files
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- Eprint ID
- 4894
- Resolver ID
- CaltechAUTHORS:GAImcb87
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