Developmental variations in the splicing pattern of transcripts from the Drosophila gene encoding myosin alkali light chain result in different carboxyl-terminal amino acid sequences
Abstract
The total sequence of the Drosophila melanogaster gene encoding the myosin light chain dissociated by alkali (MLC-ALK) has been determined. By sequence comparisons with an MLC-ALK cDNA clone and by S1 nuclease analyses, the pattern of introns and exons within the gene has been deduced. There are multiple polyadenylylation signals that can account for most of the observed heterogeneity in the lengths of mRNAs. In the 3' half of the gene, there are two alternative splicing patterns which result in mRNAs that translate to give proteins with two alternative 14 amino acid carboxyl-terminal sequences. There is developmental regulation of the selection of the above splicing sites. One splicing pattern produces an mRNA that translates into a protein used for both larval and adult musculature, whereas the other splicing pattern is used for the latter stage only.
Additional Information
© 1985 by the National Academy of Sciences. Contributed by Norman Davidson, August 31, 1984. We thank E. Vanin and P. Perlman for valuable discussions during the preparation of this manuscript. S.F. was supported by a Muscular Dystrophy Postdoctoral Fellowship and National Research Service Award 1 F32 GM08895-01. The work was supported by grants from the National Institutes of Health to N.D. and S.F. (GM33270) and from the American Cancer Society (IN-16V) to S.F. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.Attached Files
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Additional details
- PMCID
- PMC397056
- Eprint ID
- 7498
- Resolver ID
- CaltechAUTHORS:FALpnas85
- Muscular Dystrophy Association
- 1 F32 GM08895-01
- NIH Postdoctoral Fellowship
- GM33270
- NIH
- IN-16V
- American Cancer Society
- Created
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2007-02-28Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field