Plaque formation and isolation of pure lines with poliomyelitis virus
- Creators
- Dulbecco, R.
- Vogt, Marguerite
Abstract
Plaques have been produced with the three types of poliomyelitis viruses on monolayer tissue cultures of monkey kidney and monkey testis. The number of plaques was proportional to the concentration of the virus. Each plaque originates, therefore, from a single virus particle, defined as the virus unit that is unseparable by dilution. The plaques are due to the specific action of the virus since they are suppressed by type-specific antiserum. Pure virus lines were established by isolating the virus population produced in single plaques. These derived virus lines had the same morphological, serological, and pathogenic properties as the parent strain. High titer virus stocks, with titers up to 7 x 10^8 plaque-forming particles per ml., were obtained.
Additional Information
© 1954, by The Rockefeller Institute for Medical Research New York. RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. (Received for publication, June 1, 1953) Grateful acknowledgment is made to Dr. C.F. Pait for numerous discussions and for performing the intracerebral inoculation of the monkeys; to Mrs. Kathy Hayden and to Mrs. Patrida Knuth for their valuable technical assistance. Aided by a grant from The National Foundation for Infantile Paralysis, Inc.Attached Files
Published - DULjem54b.pdf
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Additional details
- PMCID
- PMC2180341
- Eprint ID
- 12238
- Resolver ID
- CaltechAUTHORS:DULjem54b
- National Foundation for Infantile Paralysis
- Created
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2008-10-30Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field