A microfabricated device for sizing and sorting DNA molecules

Creators: Chou, Hou-Pu; Spence, Charles; Scherer, Axel; Quake, Stephen R.

Abstract

We have demonstrated a microfabricated single-molecule DNA sizing device. This device does not depend on mobility to measure molecule size, is 100 times faster than pulsed-field gel electrophoresis, and has a resolution that improves with increasing DNA length. It also requires a million times less sample than pulsed-field gel electrophoresis and has comparable resolution for large molecules. Here we describe the fabrication and use of the single-molecule DNA sizing device for sizing and sorting DNA restriction digests and ladders spanning 2-200 kbp.

Additional Information

© 1999 by The National Academy of Sciences Communicated by John D. Baldeschwieler, California Institute of Technology, Pasadena, CA, October 26, 1998 (received for review August 28, 1998) We thank Wayne Volkmuth for helpful discussions and contributions in the early stages of this work. This work was supported in part by the Powell Foundation and National Institutes of Health Grant HG01641-01. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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